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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: May 8, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Conditionally fluorescent molecular probes for detecting single base changes in double-stranded DNA.

Sherry Xi Chen1, David Yu Zhang, Georg Seelig

  • 1Department of Electrical Engineering, University of Washington, Seattle, Washington 98195, USA.

Nature Chemistry
|August 23, 2013
PubMed
Summary

New DNA probes can detect single-base variations in genetic sequences. This breakthrough in nucleic acid analysis offers reliable detection for research and clinical applications, improving genetic diagnostics.

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Published on: September 25, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Distinguishing closely related nucleic acid sequences is crucial for research and clinical applications.
  • Small variations in DNA can lead to significant phenotypic changes.

Purpose of the Study:

  • To develop and demonstrate the efficacy of conditionally fluorescent DNA probes for detecting single-base variations.
  • To establish a novel mechanism for enhanced discrimination of single nucleotide polymorphisms (SNPs).

Main Methods:

  • Utilized novel, programmable, conditionally fluorescent DNA probes.
  • Engineered probes to create two destabilizing mismatch bubbles per SNP, unlike traditional single-mismatch assays.
  • Tested probe performance across a wide range of conditions and in the presence of excess non-target DNA.

Main Results:

  • Probes successfully distinguished single-base variations in target DNA.
  • A 12,000-fold excess of a target with an SNP was required to match the fluorescence of one equivalent of the intended target.
  • Demonstrated reliable detection of point mutations in the Escherichia coli rpoB gene.

Conclusions:

  • Conditionally fluorescent DNA probes offer a robust method for identifying single nucleotide polymorphisms.
  • The probe design overcomes synthesis limitations, enabling the analysis of long DNA sequences.
  • This technology has significant potential for genetic research and clinical diagnostics.