Related Experiment Video

Updated: May 8, 2026

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

Structural properties of polyphenols causing cell cycle arrest at G1 phase in HCT116 human colorectal cancer cell

Soon Young Shin1, Hyuk Yoon, Seunghyun Ahn

  • 1Department of Biological Sciences, Konkuk University, Seoul 143-701, Korea. shinsy@konkuk.ac.kr

International Journal of Molecular Sciences
|August 23, 2013
PubMed

Insights

Plant polyphenols can halt cell cycle progression at different stages. This study identifies key structural features (biophores) responsible for G1 cell cycle arrest, aiding in the design of novel chemopreventive agents.

Area of Science:

  • Phytochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Plant-derived polyphenols are investigated as potential chemopreventive agents.
  • Polyphenols exhibit varied effects on cell cycle progression, arresting it at G1 or G2/M phases through distinct mechanisms.
  • Previous research indicates that minor structural variations in polyphenols significantly alter their biological activities, but the specific structural determinants for G1 arrest are unclear.

Purpose of the Study:

  • To elucidate the structural properties of polyphenols responsible for inducing G1 cell cycle arrest.
  • To conduct a quantitative structure-activity relationship (QSAR) study on a diverse set of polyphenols.
  • To identify key biophores that mediate G1 cell cycle arrest.

Main Methods:

  • Synthesis and preparation of 27 distinct polyphenols, encompassing eight different chemical scaffolds.
  • Analysis of cell cycle profiles to determine the effects of each polyphenol.
  • Quantitative structure-activity relationship modeling to correlate polyphenol structure with G1 arrest activity.

Main Results:

  • Identification of specific biophores within polyphenol structures that are critical for inducing G1 cell cycle arrest.
  • Demonstration that distinct structural features dictate the phase of cell cycle arrest.
  • Establishment of structure-activity relationships for G1 arrest.

Conclusions:

  • The identified biophores provide crucial insights into the molecular mechanisms of G1 cell cycle arrest by polyphenols.
  • These findings will facilitate the rational design of novel polyphenols with targeted chemopreventive properties.
  • This study advances the understanding of polyphenol-induced cell cycle modulation for cancer prevention strategies.

More Related Videos

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro12:00

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Published on: July 5, 2017

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...