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Related Concept Videos

Fractures: Bone Repair01:27

Fractures: Bone Repair

Treatment for a fracture is based on the type of break, the bone affected, and the patient's age.
Minor fractures with no bone displacement are treated by immobilizing the fractured bone using a cast or splint. However, in the case of fractures with displaced bones, the broken bones are repositioned before immobilization to ensure successful healing without deformation and loss of function. The realignment of fractured bone ends is performed through a process called reduction. If the procedure...

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Updated: May 8, 2026

Fracture Apparatus Design and Protocol Optimization for Closed-stabilized Fractures in Rodents
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Hyperbaric hyperoxia accelerates fracture healing in mice.

Shigeo Kawada1, Eiji Wada, Ryoichi Matsuda

  • 1Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan. kawada@idaten.c.u-tokyo.ac.jp

Plos One
|August 23, 2013
PubMed
Summary

Hyperbaric hyperoxia (HBO) therapy boosts bone formation and accelerates fracture healing in mice. This enhanced bone metabolism occurred without negative impacts on DNA or calcium levels, suggesting a safe therapeutic potential.

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In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

Published on: November 14, 2017

Area of Science:

  • Biomedical Engineering
  • Orthopedics
  • Physiology

Background:

  • Increased oxygen tension is known to influence bone metabolism.
  • Hyperbaric hyperoxia (HBO) involves exposure to elevated oxygen levels at increased atmospheric pressure.
  • Understanding HBO's effects on bone health is crucial for therapeutic applications.

Purpose of the Study:

  • To investigate the impact of HBO on bone mineral apposition and formation rates in vivo.
  • To evaluate the efficacy of HBO in accelerating fracture healing.
  • To assess potential side effects such as oxidative DNA damage and calcium homeostasis disruption.

Main Methods:

  • Experiment 1: Mice exposed to HBO (90% O₂, 2 ATA, 90 min/day for 5 days) to measure bone formation markers.
  • Experiment 2: Femur fracture model in mice with subsequent HBO treatment (5 times/week for 6 weeks).
  • Evaluated bone mineral apposition, bone formation rates, gene expression, fracture callus size, radiographic healing, and biomechanical properties.

Main Results:

  • HBO significantly increased mineral apposition and bone formation rates in vertebrae and femurs.
  • HBO elevated mRNA expression of type 1 collagen alpha 1 and alkaline phosphatase.
  • Fracture healing was accelerated, with larger callus size and improved biomechanical properties (stiffness, maximum load) in the HBO group.

Conclusions:

  • Exposure to hyperbaric hyperoxia enhances bone anabolism.
  • HBO treatment significantly accelerates fracture healing in a mouse model.
  • These benefits were observed without inducing oxidative DNA damage or altering plasma calcium homeostasis.