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Related Experiment Videos

Trypsin inhibitors analysis: direct chromatographic titration.

G Raspi1, A Lo Moro, M Spinetti

  • 1Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Italy.

The Analyst
|May 1, 1990
PubMed
Summary

A new titrimetric method was developed to standardize human urinary trypsin inhibitors (UTIs). This technique offers a reliable alternative for quantifying these important bioclinical substances in biological fluids.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Human urinary trypsin inhibitors (UTIs) are present in plasma and biological fluids.
  • Quantifying UTIs is of bioclinical importance.

Purpose of the Study:

  • To develop a novel titrimetric method for standardizing human urinary trypsin inhibitors (UTIs).
  • To provide an alternative to traditional spectrophotometric methods.

Main Methods:

  • Direct titration of UTIs with a standardized bovine trypsin solution.
  • Monitoring residual inhibitor levels using reversed-phase high-performance liquid chromatography (RP-HPLC).

Main Results:

  • The titrimetric method demonstrated reliable standardization of three human UTIs.
  • Within-day and between-day coefficients of variation were reported.

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  • The limit of determination for each UTI was established.

    • Conclusions:

    • The developed titrimetric method is a suitable alternative for UTI standardization.
    • This method facilitates accurate quantification of UTIs in biological samples.