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Updated: May 8, 2026

Air-sampled Filter Analysis for Endotoxins and DNA Content
Published on: March 7, 2016
γ-Cyclodextrin-polyurethane copolymer adsorbent for selective removal of endotoxin from DNA solution
Masayo Sakata1, Koji Uezono, Kasane Kimura
1Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, Chuo-ku, Kumamoto 860-8555, Japan; Japan Science and Technology (JST), Core Research for Evolutional Science and Technology (CREST), Chiyoda-ku, Tokyo 102-0075, Japan.
Abstract:
Copolymer particles for removal of endotoxins (lipopolysaccharides, LPSs) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1,6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that of poly(ε-lysine)-immobilized Cellufine (cationic adsorbent) or polystyrene particles (hydrophobic adsorbent) by a batch method. When DNA was present in solution with LPSs under physiological conditions (pH 6.0, ionic strength of μ = 0.05-0.8), LPS-removing activity of the cationic or hydrophobic adsorbent was unsatisfactory because both the DNA and the LPSs were adsorbed onto each adsorbent. By contrast, the copolymer particles with γ-CyD cavity (CyD content: 14-20 mol%) could selectively remove LPSs from a DNA solution (50 μg ml(-1), pH 6.0, and μ = 0.05-0.2) containing LPSs (15 EU ml(-1)) without the adsorption of DNA. The residual concentration of LPSs in the treated DNA solution was below 0.1 EU ml(-1), and the recovery of DNA was 99%.
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