Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA methylation reprogramming in marsupial embryos is restricted to the extraembryonic lineage.

Nature communications·2026
Same author

Catalytic Enantioselective Dearomatizing [2,3]-Wittig Rearrangements Allow Divergent [2,3]-, [1,2]-, and Sommelet-Hauser-Type Products.

Journal of the American Chemical Society·2026
Same author

The acylative kinetic resolution of 1,2-azaborine naphthols.

Chemical science·2026
Same author

The catalytic enantioselective [1,2]-Wittig rearrangement cascade of allylic ethers.

Nature chemistry·2026
Same author

Effectiveness of Infliximab for Refractory Nonischemic Cerebral Enhancing Foreign-Body Granulomatous Lesions After Endovascular Therapy.

Neurology·2025
Same author

From Oxygen to Tellurium: The Impact of the Chalcogen on Nucleophilicities and Basicities of Isochalcogenourea Catalysts.

Angewandte Chemie (International ed. in English)·2025

Related Experiment Video

Updated: May 8, 2026

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
11:19

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

Published on: September 26, 2015

MLML: consistent simultaneous estimates of DNA methylation and hydroxymethylation.

Jianghan Qu1, Meng Zhou, Qiang Song

  • 1Molecular and Computational Biology, University of Southern California, Los Angeles, CA 90089, USA.

Bioinformatics (Oxford, England)
|August 24, 2013
PubMed
Summary

This study introduces a new computational method for accurately measuring 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) levels. The method integrates data from multiple sequencing techniques for consistent epigenetic analysis.

More Related Videos

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
07:19

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

Published on: February 1, 2011

Related Experiment Videos

Last Updated: May 8, 2026

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
11:19

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

Published on: September 26, 2015

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
07:19

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

Published on: February 1, 2011

Area of Science:

  • Epigenetics
  • Genomics
  • Molecular Biology

Background:

  • 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) are key epigenetic modifications in mammalian cells.
  • 5-hmC plays a role in demethylation and epigenomic regulation during development.
  • Existing methods for profiling 5-mC and 5-hmC can yield inconsistent results when combined.

Purpose of the Study:

  • To develop a robust computational method for simultaneously estimating 5-mC and 5-hmC levels.
  • To ensure consistency of epigenetic modification estimates across different sequencing experiments.
  • To provide a reliable tool for analyzing complex epigenetic landscapes.

Main Methods:

  • Jointly modeling read counts from bisulfite sequencing (BS-seq).
  • Integrating data from oxidative bisulfite sequencing (oxBS-seq).
  • Utilizing Tet-Assisted Bisulfite sequencing (TAB-seq) data.

Main Results:

  • Achieved simultaneous and consistent estimation of 5-hmC and 5-mC levels.
  • Developed a method that reconciles data from multiple epigenetic profiling techniques.
  • Provides accurate quantification of both major cytosine modifications.

Conclusions:

  • The presented method offers a unified approach for analyzing 5-mC and 5-hmC.
  • Enables more reliable epigenomic studies by ensuring cross-experimental consistency.
  • Facilitates deeper understanding of epigenetic regulation in development and disease.