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Medial septal cholinergic neurons modulate isoflurane anesthesia
Siew Kian Tai1, Jingyi Ma, L Stan Leung
1From the Department of Physiology and Pharmacology (S.K.T., J.M., L.S.L.) and Program in Neuroscience (L.S.L.), The University of Western Ontario, London, Ontario, Canada.
Anesthesiology
|August 24, 2013
Summary
Selective lesioning of septal cholinergic neurons increased sensitivity to isoflurane anesthesia in rats. This finding highlights the role of these neurons in modulating anesthetic effects, impacting both induction and emergence times.
Area of Science:
- Neuroscience
- Anesthesiology
- Cholinergic system
Background:
- Cholinergic drugs influence general anesthesia responses.
- The impact of selective cholinergic neuron lesions on volatile anesthetic sensitivity was previously unknown.
Purpose of the Study:
- To investigate the effect of selective septal cholinergic neuron lesions on isoflurane and ketamine sensitivity.
- To assess the role of these neurons in modulating anesthetic-induced loss of righting reflex (LORR).
Main Methods:
- Rats underwent selective lesioning of medial septal cholinergic neurons using 192 IgG-saporin.
- Anesthetic sensitivity to isoflurane and ketamine was measured by LORR.
- Histological analysis confirmed lesion efficacy by counting choline acetyltransferase-immunoreactive cells.
Main Results:
- Lesioned rats showed an 85% reduction in cholinergic neurons and increased sensitivity to isoflurane.
- Isoflurane LORR induction time decreased, and emergence time increased in lesioned rats.
- No significant difference in ketamine sensitivity or parvalbumin-immunoreactive cells was observed between groups.
Conclusions:
- Septal cholinergic neurons play a crucial role in modulating isoflurane anesthesia sensitivity.
- Anesthesia induction and emergence are affected by the integrity of these cholinergic pathways.
- Hippocampal gamma power changes correlate with isoflurane sensitivity and LORR.
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