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Cryopreservation of mutton snapper ( Lutjanus analis) sperm
Eduardo G Sanches1, Idili R Oliveira, Pedro C Da Silva Serralheiro
1Núcleo de Pesquisa e Desenvolvimento do Litoral Norte, Instituto de Pesca/APTA/SAA, 11680-000 Ubatuba, SP, Brasil. esanches@pesca.sp.gov.br
Anais Da Academia Brasileira De Ciencias
|August 24, 2013
Summary
Developing a semen cryopreservation protocol for mutton snapper (Lutjanus analis) involved optimizing extenders, cryoprotectant concentrations, and cooling rates. The best results combined a pH 8.2 extender, 10% dimethyl sulfoxide (DMSO), and a -60°C/min cooling rate, yielding over 59% fertilization.
Area of Science:
- Aquaculture
- Reproductive Biology
- Cryobiology
Background:
- Semen cryopreservation is crucial for aquaculture, enabling genetic resource management and breeding programs.
- Developing effective cryopreservation protocols for marine fish like mutton snapper (Lutjanus analis) is essential for sustainable aquaculture.
Purpose of the Study:
- To establish an optimized protocol for cryopreserving mutton snapper (Lutjanus analis) sperm.
- To evaluate the effects of different extenders, cryoprotectant concentrations, and cooling rates on sperm viability.
Main Methods:
- A factorial experiment analyzed three extenders (pH 6.1, 7.8, 8.2), two dimethyl sulfoxide (DMSO) concentrations (5%, 10%), and three cooling rates (-90, -60, -30°C/min).
- Semen was cryopreserved using cryogenic straws in nitrogen vapor.
- Fertilization trials assessed the viability of cryopreserved sperm.
Main Results:
- The optimal cryopreservation combination was an extender with pH 8.2, 10% DMSO, and a cooling rate of -60°C/min.
- This combination significantly improved sperm motility rate and motility duration (P < 0.05).
- Fertilization rates exceeding 59% were achieved using the cryopreserved sperm.
Conclusions:
- The developed cryopreservation protocol is effective for mutton snapper (Lutjanus analis) sperm.
- This protocol supports the potential for large-scale sperm banking and selective breeding in this species.
