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Tenascin and fibronectin expression after pulp capping with different hemostatic agents: a preliminary study
Elaine Zanchin Baldissera1, Adriana Fernandes da Silva, Ana Paula Neutzling Gomes
1Graduate Program in Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil.
Brazilian Dental Journal
|August 24, 2013
Summary
Hemostatic agents did not affect the expression of tenascin (TN) and fibronectin (FN) during pulp repair. These glycoproteins play a role in reparative dentin formation following calcium hydroxide capping.
Area of Science:
- Dental Pulp Biology
- Biomaterials Science
- Immunohistochemistry
Background:
- Pulp capping with calcium hydroxide (CH) aims to preserve pulp vitality.
- Extracellular matrix glycoproteins like tenascin (TN) and fibronectin (FN) are crucial in tissue repair and regeneration.
- Understanding their expression patterns post-CH capping under different hemostasis protocols is essential for optimizing pulp repair strategies.
Purpose of the Study:
- To investigate the expression of TN and FN in human dental pulp following capping with CH.
- To evaluate the influence of different hemostatic agents (saline, sodium hypochlorite, chlorhexidine) on TN and FN expression during pulp repair.
- To correlate the expression of TN and FN with the stages of pulp repair and dentin bridge formation.
Main Methods:
- Class I cavities with pulp exposure were created in human third molars.
- Different hemostatic agents were applied, followed by capping with CH cement.
- Immunohistochemical techniques were employed to detect TN and FN expression at 7, 30, and 90 days post-treatment.
- Formalin fixation and extraction of teeth were performed for histological analysis.
Main Results:
- Hemostatic agents did not significantly alter the expression patterns of TN and FN.
- Both TN and FN were detected throughout the pulp tissue and around collagen fibers, but not in mineralized tissues.
- TN showed positive immunostaining in predentin, while FN exhibited variable expression.
- A transient increase in TN and FN immunostaining was observed at the pulp exposure site within 7 days.
- Positive expression around the dentin barrier was noted at 30 days, and a linear expression delimiting reparative dentin at 90 days.
Conclusions:
- Hemostatic agents do not influence the expression of tenascin and fibronectin during pulp repair after CH capping.
- Tenascin and fibronectin are present in various pulp regions and at different time points, indicating their involvement in the pulp repair process.
- The spatiotemporal expression of TN and FN suggests their significant roles in the formation of reparative dentin following dental pulp capping procedures.
