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Influence of substances affecting cell sulfhydryl/disulfide status on adherence of human monocytes
S Krause1, P Arese, S Heptinstall
1Institute of Pathological Biochemistry, Medical Academy, Erfurt, German Democratic Republic.
Abstract:
An in-vitro model is described for investigating the adherence of human monocytes. Mononuclear cells (MNC) isolated from peripheral blood were incubated in plastic multiwell cell culture plates. The adherence of monocytes was quantified on the basis of their DNA content. The cell adherence proceeds rapidly; after 30 min of incubation about 85% of maximum adherent cells were attached to the plastic surface. On testing the effects of various sulfhydryl-affecting substances on monocyte adherence we found that the thiol-oxidizing compound diamide (0.1-1 mmol/l) inhibited the adherence by about 60%. Incubation with an extract of the plant feverfew, which contains materials that neutralize cellular sulfhydryl groups, also diminished monocyte adherence. The soluble thiol 2-mercaptopropionylglycine, in a concentration range of 0.5 to 10 mmol/l, had no effect on adherence in this system. The results suggest that cellular sulfhydryl groups play an important role in the adherence of monocytes.
Insights
Cellular sulfhydryl groups are crucial for human monocyte adherence to surfaces. Inhibiting these groups with compounds like diamide significantly reduces monocyte attachment in vitro.
Area of Science:
- Immunology
- Cell Biology
Background:
- Monocyte adherence is a critical step in inflammatory and immune responses.
- Understanding the molecular mechanisms regulating monocyte adhesion is essential for developing targeted therapies.
Purpose of the Study:
- To investigate the role of cellular sulfhydryl groups in human monocyte adherence.
- To establish an in vitro model for quantifying monocyte adherence.
Main Methods:
- Isolation of mononuclear cells (MNC) from peripheral blood.
- Incubation of MNC in multiwell plates to assess monocyte adherence.
- Quantification of adherence based on DNA content.
- Testing the effects of sulfhydryl-affecting substances, including diamide and feverfew extract.
Main Results:
- Monocyte adherence occurred rapidly, with 85% attachment within 30 minutes.
- Diamide (0.1-1 mmol/l) inhibited monocyte adherence by approximately 60%.
- Feverfew extract, known to neutralize sulfhydryl groups, also diminished adherence.
Conclusions:
- Cellular sulfhydryl groups play a significant role in regulating human monocyte adherence.
- Targeting sulfhydryl groups may offer a strategy to modulate monocyte-mediated inflammatory processes.