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Updated: May 8, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

hClock gene expression in human colorectal carcinoma.

Liying Wang¹, Bozan Chen, Yaping Wang

¹Clinical Skills Learning Center, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.

Molecular Medicine Reports

|August 24, 2013

Summary

The human Clock (hClock) gene is highly expressed in colorectal cancer tissues, correlating with advanced tumor stages and metastasis. This suggests hClock plays a role in CRC development and progression.

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Last Updated: May 8, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

Area of Science:

Molecular Biology
Oncology
Chronobiology

Background:

Circadian rhythm disruption is implicated in colorectal cancer (CRC) initiation and progression.
Molecular alterations in circadian genes within CRCs remain underexplored.

Purpose of the Study:

To investigate human Clock (hClock) gene and protein expression changes in colorectal carcinomas (CRCs).
To explore the potential role of hClock in CRC development and metastasis.

Main Methods:

Immunofluorescence and quantitative polymerase chain reaction (qPCR) were used to analyze hCLOCK protein and gene expression.
Expression levels were compared between 30 CRC tissues and paired non-cancerous tissues.
Correlations between hClock expression and clinicopathological features, including lymph node metastasis, were assessed.

Main Results:

hCLOCK protein and hClock gene were expressed in all CRC specimens.
Significantly higher hClock expression was observed in CRC tissues compared to non-cancerous tissues.
Elevated hClock expression correlated with poorly differentiated tumors, late-stage disease, and lymph node metastasis.
Strong positive correlations were found between hClock expression and ARNT, HIF-1α, and VEGF expression.

Conclusions:

The circadian gene hClock is upregulated in colorectal carcinomas.
hClock expression is linked to advanced tumor characteristics and metastatic potential.
hClock may promote CRC angiogenesis and metastasis through interaction with HIF-1α/ARNT and activation of VEGF.