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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Updated: May 8, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

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Published on: September 3, 2013

A negative dielectrophoresis and gravity-driven flow-based high-throughput and high-efficiency cell-sorting system.

Dongkyu Lee1, Dowon Kim, Youngwoong Kim

  • 11School of Aerospace and Mechanical Engineering, Nano Bio Robotics Lab, Korea Aerospace University, Korea.

Journal of Laboratory Automation
|August 24, 2013
PubMed
Summary

This study introduces a novel negative dielectrophoresis (n-DEP) system for efficient cell separation. The high-throughput system achieves 95% separation efficiency for live K562 cells from dead K562 cells.

Keywords:
cantilever-type electrode (CE) arrayhigh-throughput sorting (HTS)macro-sized channelnegative dielectrophoresisseparation

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Published on: January 20, 2023

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Separation

Background:

  • Cell separation is crucial in various biological and medical applications.
  • Existing methods often face limitations in throughput and efficiency.
  • Negative dielectrophoresis (n-DEP) offers a promising label-free approach for cell manipulation.

Purpose of the Study:

  • To develop and validate a high-throughput and high-efficiency cell separation system utilizing negative dielectrophoresis (n-DEP).
  • To optimize separation conditions by analyzing hydrodynamic and n-DEP forces.
  • To demonstrate the system's capability in separating live from dead cells.

Main Methods:

  • Design of a macro-sized channel with cantilever-type electrode (CE) arrays for n-DEP force generation.
  • Implementation of double separation modules for enhanced efficiency.
  • Precise control of hydrodynamic forces using flow regulators at each outlet.
  • Theoretical analysis of forces acting on cells and experimental validation of separation conditions.

Main Results:

  • The n-DEP system utilizes macro-sized channels and CE arrays (150 µm × 500 µm × 50 µm).
  • Double separation modules and flow regulators ensure high efficiency and precise control.
  • Separation of live K562 from dead K562 cells achieved 95% efficiency at low voltage (7Vp-p, 100 kHz) and specific flow rates.

Conclusions:

  • The developed n-DEP system provides a robust platform for high-throughput and high-efficiency cell separation.
  • The system demonstrates significant potential for applications requiring precise separation of viable and non-viable cells.
  • Optimization of hydrodynamic and n-DEP forces is key to achieving high separation performance.