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Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
Published on: May 21, 2012
Dysregulated circulating dendritic cell function in ulcerative colitis is partially restored by probiotic strain
Elizabeth R Mann1, Jialu You, Verena Horneffer-van der Sluis
1Antigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark's Campus, Level 7W St. Mark's Hospital, Watford Road, Harrow HA1 3UJ, UK.
Probiotic Lactobacillus casei Shirota (LcS) restores dendritic cell (DC) function in ulcerative colitis (UC) patients. LcS treatment may promote immune homeostasis, offering therapeutic potential for UC by modulating DC immune responses.
Area of Science:
- Immunology
- Microbiology
- Gastroenterology
Background:
- Dendritic cells (DCs) are crucial in immune responses and implicated in ulcerative colitis (UC) pathogenesis.
- Investigating the immunomodulatory effects of Lactobacillus casei Shirota (LcS) on human DCs from healthy individuals and UC patients.
Purpose of the Study:
- To determine the impact of Lactobacillus casei Shirota (LcS) on dendritic cell (DC) function in ulcerative colitis (UC).
- To assess LcS's ability to modulate DC-T cell interactions and cytokine production in UC patients.
Main Methods:
- Human peripheral blood DCs from healthy controls and UC patients were conditioned with heat-killed LcS.
- Conditioned DCs were used to stimulate allogeneic T cells in a mixed leucocyte reaction.
Main Results:
- UC-derived DCs showed reduced T cell stimulatory capacity and increased skin-homing markers (CLA, CCR4) compared to controls.
- LcS treatment normalized UC-DC stimulatory capacity and induced a multihoming profile in control DCs.
- LcS enhanced TGFβ production by T cells in controls but not in UC patients.
Conclusions:
- Ulcerative colitis is associated with systemic DC dysfunction, potentially explaining cutaneous manifestations.
- LcS exhibits state-dependent immunoregulatory activities, suggesting a role in promoting immune homeostasis for UC therapy.
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