Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Studies on peroxisomal membranes.

D Hardeman1, C Versantvoort, J M van den Brink

  • 1Centre for Biomembranes and Lipid Enzymology, University of Utrecht, Utrecht, The Netherlands.

Biochimica Et Biophysica Acta
|August 24, 1990
PubMed
Summary

Researchers quantified phospholipid to protein ratios in rat liver organelles. Triton X-114 phase separation effectively isolated peroxisomal membrane proteins, offering a new method for studying organelle membranes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lifetime-Limited and Tunable Emission from Single Charge-Stabilized Nickel Vacancy Centers in Diamond.

Physical review letters·2025
Same author

Long-term observations on the hydraulic performance of a combined capillary barrier-methane oxidation landfill cover system.

Waste management (New York, N.Y.)·2024
Same author

Physiologically based pharmacokinetic modeling in regulatory decision-making at the European Medicines Agency.

Clinical pharmacology and therapeutics·2016
Same author

Immunohistochemical and serological investigation of experimental Ornithobacterium rhinotracheale infection in chickens.

Avian pathology : journal of the W.V.P.A·2016
Same author

[Genome composition analysis of the reassortant influenza viruses used in seasonal and pandemic live attenuated influenza vaccine].

Molekuliarnaia genetika, mikrobiologiia i virusologiia·2012
Same author

Three-dimensional quantification of regional left-ventricular dyssynchrony by magnetic resonance imaging.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2012

Area of Science:

  • Cell Biology
  • Biochemistry
  • Membrane Biology

Background:

  • Understanding the composition of cellular membranes is crucial for elucidating organelle function.
  • Rat liver peroxisomes, mitochondria, and microsomes possess distinct phospholipid and protein profiles.

Purpose of the Study:

  • To determine the phospholipid/protein ratios in rat liver peroxisomes, mitochondria, and microsomes.
  • To evaluate sodium carbonate and Triton X-114 phase separation methods for isolating membrane components and preserving enzymatic activity.

Main Methods:

  • Determination of phospholipid/protein ratios in isolated organelle fractions.
  • Treatment with sodium carbonate to pellet membrane fragments.
  • Triton X-114 phase separation to isolate peroxisomal membrane components.

Related Experiment Videos

  • Enzymatic activity assays for integral membrane and matrix proteins.

    • Main Results:

    • Initial phospholipid/protein ratios varied among organelles, with peroxisomes showing 153 nmol/mg after correction.
    • Sodium carbonate treatment disrupted enzymatic activity, while Triton X-114 phase separation yielded a membrane fraction enriched in proteins and phospholipids.
    • Triton X-114 effectively separated integral membrane proteins (over 90% recovery) from soluble matrix proteins, although peroxisomal core protein urate oxidase associated with membranes.

    Conclusions:

    • Triton X-114 phase separation is a valuable method for isolating integral membrane proteins from peroxisomes, preserving enzymatic activity.
    • This technique offers an alternative to existing methods for studying organelle membrane composition and function.