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In vitro systems for inducers of cartilage and bone development
1Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
Abstract:
Murine condylar cartilages of perinatal mice were cultured in the form of organ or tissue cultures on top of collagen sponges in medium containing 10% fetal calf serum. Cultures were treated with increasing concentrations of recombinant human growth hormone, recombinant IGF-I, insulin, TGF beta-1, FGF and recombinant fragments of human parathyroid hormone. All the above agents [except for the fragments rPTH (28-48) and (53-84)] were found to be mitogenic as they accelerated the proliferative activity of the condylar stem cells. IGF-I and insulin also stimulated the synthesis of cartilage proteoglycans. Growth hormone also supported de novo osteogenesis in vitro.