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Related Experiment Video

Updated: May 8, 2026

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Analysis of ¹⁸FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Published on: September 5, 2017

Tuberculosis: feeding the enemy.

Natalie J Garton¹, Helen M O'Hare

¹Department of Infection, Immunity and Inflammation, University of Leicester Medical School, Leicester LE1 9HN, UK.

Chemistry & Biology

|August 27, 2013

Summary

Researchers identified the nutrients and metabolic pathways utilized by intracellular Mycobacterium tuberculosis. This study used 13C labeling and novel data analysis to uncover how this bacterium sustains itself within host cells.

Area of Science:

Microbiology
Biochemistry
Cellular Biology

Background:

Intracellular Mycobacterium tuberculosis poses a significant challenge to human health.
Understanding the metabolic requirements of M. tuberculosis within host cells is crucial for developing new treatments.
Previous methods for studying intracellular bacterial nutrition have been limited.

Purpose of the Study:

To directly identify the specific nutrients M. tuberculosis utilizes for growth and survival inside host cells.
To elucidate the metabolic pathways employed by intracellular M. tuberculosis.
To overcome the experimental difficulties in studying intracellular bacterial metabolism.

Main Methods:

Utilized 13C-labeled nutrients to trace metabolic flux within M. tuberculosis.

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Published on: August 16, 2021

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Last Updated: May 8, 2026

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Analysis of ¹⁸FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Published on: September 5, 2017

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Published on: August 16, 2021

Developed and applied a novel data analysis approach to interpret labeling patterns.

Investigated M. tuberculosis within its intracellular environment.

Main Results:

Provided direct evidence of the nutrients consumed by intracellular M. tuberculosis.
Identified key metabolic pathways active during intracellular growth.
Demonstrated the efficacy of the new analytical method for studying bacterial metabolism.

Conclusions:

The study successfully characterized the nutritional landscape of intracellular M. tuberculosis.
The findings offer new targets for anti-tuberculosis drug development.
The novel methodology can be applied to study other intracellular pathogens.