Related Experiment Video
Updated: May 8, 2026
09:36
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
MALAT-1 interacts with hnRNP C in cell cycle regulation
Feng Yang1, Fan Yi, Xiaorui Han
1Institute of Molecular Medicine and State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100871, China.
FEBS Letters
|August 27, 2013
Summary
The MALAT-1 gene transcript moves to the cytoplasm during the G2/M cell cycle phase, interacting with hnRNP C protein. Down-regulating MALAT-1 disrupts this translocation, causing cell cycle arrest.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- MALAT-1 is a conserved non-coding RNA gene.
- MALAT-1 transcripts are predominantly localized in the nucleus.
Purpose of the Study:
- To investigate the role of MALAT-1 in cell cycle regulation.
- To characterize the interaction between MALAT-1 and hnRNP C protein during the cell cycle.
Main Methods:
- Localization studies of MALAT-1 transcripts during the cell cycle.
- Investigation of MALAT-1 interaction with hnRNP C protein.
- Loss-of-function assays to assess the impact of MALAT-1 down-regulation.
Main Results:
- MALAT-1 transcripts translocate from the nucleus to the cytoplasm during the G2/M cell cycle phase.
- MALAT-1 interacts with hnRNP C protein during this cytoplasmic translocation.
- Down-regulation of MALAT-1 expression inhibits hnRNP C cytoplasmic translocation and causes G2/M cell cycle arrest.
Conclusions:
- MALAT-1 plays a critical role in regulating the G2/M cell cycle transition.
- The interaction between MALAT-1 and hnRNP C is essential for proper cell cycle progression.
Related Concept Videos
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
01:28The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...