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Related Experiment Video

Updated: May 8, 2026

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Probing bacterial metabolism during infection using high-resolution transcriptomics.

Peter Jorth1, Urvish Trivedi, Kendra Rumbaugh

  • 1Section of Molecular Genetics and Microbiology, Institute of Cell and Molecular Biology, The University of Texas at Austin, Austin, Texas, USA.

Journal of Bacteriology
|August 27, 2013
PubMed
Summary
This summary is machine-generated.

This study reveals distinct metabolic pathways for Aggregatibacter actinomycetemcomitans in vivo. Key enzymes like formate dehydrogenase H and fumarate reductase are crucial for pathogen survival and colonization.

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Area of Science:

  • Microbiology
  • Genomics
  • Pathogen Metabolism

Background:

  • Infectious diseases rely on microbial proliferation within hosts.
  • Understanding pathogen metabolic requirements for colonization is limited.
  • Aggregatibacter actinomycetemcomitans is an opportunistic pathogen.

Purpose of the Study:

  • To characterize the transcriptome of A. actinomycetemcomitans during in vivo growth.
  • To identify metabolic pathways essential for pathogen fitness in the host.
  • To compare in vivo and in vitro gene expression profiles.

Main Methods:

  • RNA sequencing (RNA-seq) for high-resolution transcriptome analysis.
  • In vivo and in vitro (biofilm) growth models.
  • Mutational analyses of differentially regulated genes.

Main Results:

  • Identified 691 transcriptional start sites and 210 noncoding RNAs in vivo.
  • Approximately 14% of A. actinomycetemcomitans genes were differentially regulated in vivo compared to in vitro biofilms.
  • Genes involved in metabolic pathways showed significant differential regulation in vivo.
  • Formate dehydrogenase H and fumarate reductase were identified as critical for in vivo fitness.

Conclusions:

  • A. actinomycetemcomitans exhibits distinct in vivo metabolism compared to in vitro growth.
  • Specific metabolic enzymes are vital for pathogen colonization and persistence.
  • This study provides a high-resolution genomic view and insights into pathogen survival strategies.