Identification of common, non-typable and autoagglutinating Salmonella strains with Premi®Test Salmonella assay

Since Salmonella serotyping according to the White-Kauffmann-Le Minor scheme fails to identify rough and atypical serological variants, this study aimed at improving serovar identification with the commercially available Premi®Test Salmonella Assay. The array was validated against a set of Salmonella reference strains (n = 27) and field isolates of known serological structure (n = 112) showing up to 97.8% congruent results. Its diagnostic suitability was further verified with random field isolates (n = 52; 100% congruence). For 'rough' isolates (n = 54) and those with antigen expression failure (n = 19) the assay showed, respectively, 98.1% and 73.7% of serovar recognition. It considerably improved diagnostic capacity while typing troublesome isolates such as those failing to express flagellar antigens or showing autoagglutination. The method offers lower labour time compared to the traditional serotyping and does not require a broad range of diagnostic sera.