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Updated: May 8, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
Published on: May 31, 2013
RNA secondary structure diagrams for very large molecules: RNAfdl
Nikolai Hecker1, Tim Wiegels, Andrew E Torda
1Centre for non-coding RNA in Technology and Health and Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark, European Molecular Biology Laboratory-Hamburg Outstation, c/o DESY, 22603 Hamburg, Germany and Centre for Bioinformatics, Institute for Biochemistry and Molecular Biology, University of Hamburg, 20146 Hamburg, Germany.
Summary:
There are many programs that can read the secondary structure of an RNA molecule and draw a diagram, but hardly any that can cope with 10(3) bases. RNAfdl is slow but capable of producing intersection-free diagrams for ribosome-sized structures, has a graphical user interface for adjustments and produces output in common formats.
Availability And Implementation:
Source code is available under the GNU General Public License v3.0 at http://sourceforge.net/projects/rnafdl for Linux and similar systems or Windows using MinGW. RNAfdl is implemented in C, uses the Cairo 2D graphics library and offers both command line and graphical user interfaces.
Contact:
hecker@rth.dk
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