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Updated: May 8, 2026
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Published on: March 31, 2016
Isolation and culture of porcine embryonic stem cells
Ivan Vassiliev1, Mark B Nottle
1Reproductive Biotechnology Group, School of Paediatrics and Reproductive Health and Centre for Stem Cell Research, Robinson Institute, The University of Adelaide, Adelaide, SA, Australia.
Abstract:
Despite their agricultural and biomedical importance, embryonic stem cells (ESCs) are yet to be isolated for the pig or the domestic ungulates in general. This suggests that methods which have been used successfully in mice may not be applicable to these. In this chapter we describe a new method for the isolation of porcine ESCs. This method differs from those described previously in that it produces homogeneous outgrowths from undifferentiated inner cell mass cells when embryos are plated onto inactivated mouse embryonic feeder layers.