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Related Experiment Video

Updated: May 8, 2026

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
10:34

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

Published on: August 7, 2013

Mouse enteric neuronal cell culture.

Yonggang Zhang1, Wenhui Hu

  • 1Department of Neuroscience, Temple University School of Medicine, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 27, 2013
PubMed
Summary
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Researchers developed two methods to culture enteric neurons from mouse guts. These techniques provide a valuable model for studying gastrointestinal functions and related disorders.

Area of Science:

  • Neuroscience
  • Gastroenterology

Background:

  • The enteric nervous system (ENS) comprises intrinsic neurons regulating gastrointestinal functions.
  • Culturing enteric neurons is crucial for studying their physiology, electrophysiology, and pharmacology.

Purpose of the Study:

  • To describe two effective methods for obtaining sufficient enteric neurons from mouse myenteric plexuses.
  • To provide a model system for research on the ENS and gut functions.

Main Methods:

  • Directly culturing primary enteric neurons isolated from mouse myenteric plexuses.
  • Inducing neuronal differentiation from enteric neural stem/progenitor cells (ENSPCs).

Main Results:

  • Both methods yield sufficient numbers of enteric neurons for experimental studies.

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Establishing Mixed Neuronal and Glial Cell Cultures from Embryonic Mouse Brains to Study Infection and Innate Immunity
07:41

Establishing Mixed Neuronal and Glial Cell Cultures from Embryonic Mouse Brains to Study Infection and Innate Immunity

Published on: June 30, 2023

Related Experiment Videos

Last Updated: May 8, 2026

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
10:34

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

Published on: August 7, 2013

Establishing Mixed Neuronal and Glial Cell Cultures from Embryonic Mouse Brains to Study Infection and Innate Immunity
07:41

Establishing Mixed Neuronal and Glial Cell Cultures from Embryonic Mouse Brains to Study Infection and Innate Immunity

Published on: June 30, 2023

  • The described techniques facilitate the establishment of a reliable model for ENS research.

    • Conclusions:

    • Two distinct methods for culturing enteric neurons are presented, offering flexibility for researchers.
    • These culture techniques are vital for advancing the understanding of gastrointestinal physiology and disease.