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Updated: May 8, 2026

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

Published on: September 10, 2009

Compartmentalized neuronal cultures.

Armine Darbinyan¹, Paul Pozniak, Nune Darbinian

¹Department of Pathology, The Mount Sinai Medical Center, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|August 27, 2013

Summary

The AXon Investigation System (AXIS™) enables spatial isolation of neuronal compartments. This microfluidic device facilitates studying compartment-specific effects of compounds on neuron function.

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Area of Science:

Neuroscience
Cell Biology
Biotechnology

Background:

Neuronal cultures exhibit random outgrowth, complicating the study of distinct cellular compartments.
Understanding the roles of somas, axons, and dendrites is crucial for deciphering complex neuronal functions and molecular mechanisms.

Purpose of the Study:

To introduce and evaluate the AXon Investigation System (AXIS™) for spatially isolating neuronal processes.
To enable compartment-specific studies of neuronal function and molecular mechanisms.

Main Methods:

Utilized the AXon Investigation System (AXIS™), a slide-mounted microfluidic device.
The system features microgrooves (10 μm width, 5 μm height) preventing cell passage but allowing neurite extension.
Established hydrostatic gradients to manipulate fluidics within the chambers.

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Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures

Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures

Published on: October 17, 2008

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Last Updated: May 8, 2026

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

Published on: September 10, 2009

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

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Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures

Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures

Published on: October 17, 2008

Main Results:

The AXIS™ device successfully oriented neuronal outgrowth and spatially isolated neuronal processes from cell bodies.
Microgroove dimensions effectively restricted cell body migration while permitting neurite extension.
The microfluidic design supported the creation of hydrostatic gradients for targeted compound application.

Conclusions:

The AXIS™ provides a robust platform for the spatial isolation of neuronal compartments.
This method allows for the investigation of compartment-specific effects of compounds on neuronal function.
Facilitates detailed studies into the molecular mechanisms governing biological processes within neuronal somas, axons, and dendrites.