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Related Experiment Video

Updated: May 8, 2026

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Published on: February 5, 2015

Synapto-protective drugs evaluation in reconstructed neuronal network.

Bérangère Deleglise¹, Benjamin Lassus, Vaneyssa Soubeyre

¹Neurobiologie des Processus Adaptatifs, CNRS, UMR7102, Paris, France ; Université Pierre et Marie Curie, Paris, France.

|August 27, 2013

Summary

A novel "brain on chip" platform enables studying synaptic collapse in neurodegenerative diseases. It identifies NAD+ and Y27632 as promising synapto-protective drugs, crucial for developing new therapies.

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Last Updated: May 8, 2026

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Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line

Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line

Published on: August 9, 2014

Area of Science:

Neuroscience
Biotechnology
Drug Discovery

Background:

Neurodegenerative diseases like Alzheimer's and Parkinson's involve early synaptic collapse, leading to cognitive decline.
Current neuroprotective strategies often fail to prevent cognitive impairment as they do not target synapses.
Lack of realistic neuronal network models hinders the development of synapto-protective drugs.

Purpose of the Study:

To introduce a microfluidic platform for studying axonal trauma in neuronal networks.
To evaluate the effects of local pathological stresses and potential protective molecules on neuronal compartments.
To screen for drugs with synapto-protective potential.

Main Methods:

Development of a microfluidic platform to reconstruct oriented mouse neuronal networks.
Individual chemical addressing of neuronal populations and sub-compartments.
Evaluation of somatic, axonal, and synaptic effects of insults and drug treatments.

Main Results:

Presynaptic loss was identified as the earliest event following axotomy, preceding axonal fragmentation.
The platform successfully demonstrated the synapto-protective effects of NAD+ and Y27632.
Resveratrol and zVAD-fmk did not prevent presynaptic degeneration in this model.

Conclusions:

The microfluidic platform is a versatile tool for fundamental neuroscience research.
This 'brain on chip' model facilitates the screening of axon- and synapto-protective drugs.
The findings highlight the importance of targeting synaptic integrity for treating neurodegenerative conditions.