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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Annexin A6 is down-regulated through promoter methylation in gastric cancer

Xian Wang1, Shengjie Zhang, Jianbin Zhang

  • 1Department of Medical Oncology, Laboratory of Cancer Biology, Institute of Clinical Science, Sir Runrun Shaw Hospital, Medical School of Zhejiang University Hangzhou, China.

American Journal of Translational Research
|August 27, 2013
PubMed
Abstract

Insights

Annexin A6 (ANXA6) acts as a tumor suppressor in gastric cancer by inhibiting Ras/MAPK signaling. Its silencing via promoter methylation, influenced by YY1, is a key event in cancer development but not a prognostic indicator.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Aberrant Ras/MAPK signaling is common in human cancers.
  • Epigenetic silencing of pathway inhibitors contributes to Ras/MAPK activation.
  • ANXA6's role in Ras inhibition in breast cancer suggests broader involvement in human cancers.

Purpose of the Study:

  • To investigate the role of ANXA6 in gastric cancer.
  • To determine if ANXA6 is epigenetically silenced in gastric cancer.
  • To explore the mechanism of ANXA6 silencing and its functional impact.

Main Methods:

  • Real-time PCR for ANXA6 expression analysis.
  • Methylation-specific PCR and bisulfite sequencing for promoter methylation.
  • Colony formation assays and western blotting for functional studies.

Main Results:

  • ANXA6 is downregulated in gastric cancer due to promoter methylation.
  • ANXA6 re-expression inhibits gastric cancer cell growth and Ras/MAPK activity.
  • Yin Yang 1 (YY1) promotes ANXA6 promoter methylation.
  • ANXA6 methylation is not associated with clinical parameters or patient survival.

Conclusions:

  • ANXA6 is epigenetically silenced by promoter methylation in gastric cancer, with YY1 involvement.
  • ANXA6 functions as a tumor suppressor by inhibiting Ras/MAPK signaling.
  • ANXA6 methylation status does not predict prognosis in gastric cancer patients.
Keywords:
ANXA6Ras signalinggastric cancermethylation

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