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Related Experiment Video

Updated: May 8, 2026

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection
03:33

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

Published on: November 17, 2023

Gold nanoparticles paper as a SERS bio-diagnostic platform.

Ying Hui Ngo1, Whui Lyn Then, Wei Shen

  • 1BioPRIA, Australian Pulp and Paper Institute (APPI), Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.

Journal of Colloid and Interface Science
|August 28, 2013
PubMed
Summary

This study presents a novel gold nanoparticle (AuNP) paper platform for sensitive and selective antibody-antigen detection using Surface Enhanced Raman Scattering (SERS). The generic biosensor effectively addresses key limitations in bioactive paper development for diagnostics.

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Area of Science:

  • Biomaterials Science
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Bioactive papers face limitations in sensitivity, selectivity, simplicity, and strength (4S).
  • Gold nanoparticles (AuNPs) on paper substrates offer potential as Surface Enhanced Raman Scattering (SERS) active materials to overcome these challenges.
  • Developing generic SERS platforms for biomolecule detection is crucial for advanced diagnostics.

Purpose of the Study:

  • To develop a generic SERS platform on AuNP-treated paper for antibody-antigen detection.
  • To functionalize the AuNP paper substrate using a streptavidin-biotin system for biomolecule immobilization.
  • To demonstrate the platform's capability in detecting antigen-antibody interactions via SERS.

Main Methods:

  • Functionalization of AuNP-treated paper with streptavidin using biotinylated-thiol.
Keywords:
AntibodyAntigenBiotinGold nanoparticles (AuNPs)PaperPaper bioassayStreptavidinSurface Enhanced Raman Scattering (SERS)

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Last Updated: May 8, 2026

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection
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  • Immobilization of biotinylated antibodies onto the streptavidin-coated paper.
  • Detection of antibody-antigen binding events using SERS spectroscopy.
  • Analysis of spectral shifts and band intensities to confirm molecular interactions and antigen presence.

    • Main Results:

    • Successful immobilization of streptavidin and antibodies confirmed by SERS spectra.
    • Detection of antibody-antigen binding through characteristic Raman band shifts (α-helix, β-sheet) and residue signals (tryptophan, tyrosine).
    • SERS signal intensity correlated positively with antigen concentration, demonstrating quantitative detection.
    • Stable retention of AuNPs on the paper substrate throughout functionalization and washing steps.

    Conclusions:

    • AuNP-treated paper serves as a robust and generic SERS platform for bio-diagnostic applications.
    • The streptavidin-biotin functionalization strategy enables versatile biomolecule immobilization.
    • The platform offers a low-cost, sensitive, and selective approach for detecting specific antigen-antibody interactions.