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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

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Published on: October 1, 2016

Colorimetric and fluorometric dual-readout sensor for lysozyme.

Hanye Zheng¹, Suyan Qiu, Kefeng Xu

¹MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China. zylin@fzu.edu.cn.

|August 28, 2013

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A novel dual-readout sensor detects lysozyme using colorimetric and fluorometric methods. This sensitive assay offers a reliable way to quantify lysozyme in various samples.

Area of Science:

Biochemical Sensing
Analytical Chemistry
Nanomaterials

Background:

Lysozyme is a crucial enzyme in biological systems.
Accurate lysozyme detection is vital for diagnostics and food safety.
Existing detection methods may lack sensitivity or selectivity.

Purpose of the Study:

To develop a novel, highly sensitive, and selective dual-readout sensor for lysozyme detection.
To utilize fluorescence resonance energy transfer (FRET) for signal generation.
To validate the sensor's performance in complex matrices like hen egg white.

Main Methods:

Design of a sensor based on triazolylcoumarin molecules and gold nanoparticles (AuNPs).
Exploitation of FRET for fluorescence quenching and subsequent recovery upon lysozyme binding.

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Colorimetric and fluorometric readout for dual detection.

Determination of the limit of detection (LOD) and lowest detectable concentration.

Main Results:

The sensor exhibited dual-readout capabilities (colorimetric and fluorometric).
Lysozyme binding to AuNPs induced fluorescence recovery and a visible color change from red to purple.
The lowest detectable concentration was 50 ng mL⁻¹ (naked eye) and LOD was 23 ng mL⁻¹ (fluorescence).
Successful lysozyme detection was demonstrated in hen egg white samples.

Conclusions:

A novel, sensitive, and selective dual-readout sensor for lysozyme was successfully developed.
The sensor offers a reliable and efficient method for lysozyme determination.
The findings support the sensor's potential application in biological and food safety analyses.