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Updated: May 8, 2026

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory
Published on: July 10, 2018
Ecotoxicity of pp'DDE to Daphnia magna
Roberta Bettinetti1, Valeria Croce, Francesca Noè
1Theoretical and Applied Science Department, University of Insubria, Via Dunant 3, Varese, Italy, roberta.bettinetti@uninsubria.it.
Abstract:
pp'-Dichlorodiphenyl-dichloroethylene (pp'DDE), a metabolite of pp'-dichlorodiphenyl-trichloroethane poses a risk for many ecosystems in spite of the banning of the parent compound because of its persistence and bioaccumulability. Nevertheless, the knowledge of acute and chronic toxicity on aquatic organisms is still very poor. In the present study, Daphnia magna was exposed to varying concentrations of pp'DDE in water and through diet to determine both acute toxicity and potential for effects on reproduction and survivability. The 48 h IC50 was 5.08 μg L(-1) (3.76-7.01 μg L(-1)). As pp'DDE concentration in water was not stable and the amount assumed by food cannot be established with certainty, the results of chronic toxicity tests were expressed as the concentration in the organism which caused a negative effect. Grazing activity was affected with a pp'DDE concentration in the organism of 24 ng mg(-1) d.w., while the lowest observed effect concentration for fecundity reduction was 109 ng mg(-1) d.w.
