Related Experiment Video
Updated: May 8, 2026

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS
Published on: November 8, 2024
Validation of blood and liver oxymorphone analysis using LC-MS-MS: concentrations in 30 fatal overdoses
Erin D Crum1, Kristen M Bailey, Lauren L Richards-Waugh
11 Marshall University, 1401 Forensic Science Drive, Huntington, WV 25701, USA.
Abstract:
A sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the quantitation of oxymorphone (OM) in human whole blood and liver. Sample preparation was done by solid-phase extraction, using deuterated OM as the internal standard. Separation was achieved using a Waters Aquity UPLC HSS T3 column. Analysis utilized positive electrospray ionization and multiple reaction monitoring. As part of the validation, studies were conducted to determine potential interference, selectivity, ion suppression/enhancement and carryover. Calibration model, limit of detection (LOD), lower limit of quantitation (LLOQ), precision and accuracy were also established. The linear range of the method was 2-500 ng/mL in blood and 5-500 ng/g in the liver. The LOD and LLOQ were 2 ng/mL for blood and 5 ng/g for the liver. Blood and/or liver specimens from 30 cases were analyzed. OM concentrations ranged from 23 to 554 ng/mL ( , n = 26) in blood and 48 to 1740 ng/g ( , n = 30) in the liver.
More Related Videos
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
11:06Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia
Published on: April 7, 2023
Related Concept Videos
Drug Concentrations: Measurements
Plasma —...
Opioid Analgesics: Synthetic and Semisynthetic Opioids
Drug Toxicity: Risk factors
Pharmaceutical Poisoning: Potential Scenarios
Therapeutic Drug Monitoring: Drug Analysis Methods
Opioid Analgesics: Morphine and Other Natural Cogeners