Factors affecting production and activity of phospholipase C by Yersinia enterocolitica

The production of phospholipase C by Yersinia enterocolitica strain SG was optimum at 37 degrees C at pH 6.5. No enzyme activity could be detected when the organism was grown at extreme pH values (pH greater than 8.5 or less than 5.0). The enzyme production was maximum when the organism was grown under static conditions in TSB medium. All solvents and salts inhibited the enzyme activity, whereas loss of activity was 95% in presence of methanol (20%) and 99% in presence of sodium azide (0.2 mol/l). The enzyme activity was increased twofold in the presence of cysteine and decreased by 98% in the presence of sodium perchlorate (0.2 mol/l).