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Reduction in Enteroccocus faecalis counts - a comparison between rotary and reciprocating systems.

C M Ferrer-Luque1, I Bejarano, M Ruiz-Linares

  • 1Department of Stomatology, School of Dentistry, University of Granada, Granada, Spain.

International Endodontic Journal
|August 29, 2013
PubMed
Summary

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Rotary and reciprocating root canal systems show similar initial reduction of Enterococcus faecalis. However, the Mtwo system demonstrated superior long-term disinfection against bacterial regrowth.

Area of Science:

  • Endodontics
  • Microbiology
  • Dental Materials Science

Background:

  • Enterococcus faecalis is a common pathogen in persistent root canal infections.
  • Effective chemomechanical preparation is crucial for eliminating intraradicular bacteria.
  • Comparing rotary and reciprocating systems for Enterococcus faecalis reduction is essential for clinical practice.

Purpose of the Study:

  • To compare the efficacy of rotary (Mtwo, Twisted File) and reciprocating (WaveOne) systems in reducing Enterococcus faecalis.
  • To evaluate the impact of distilled water versus sodium hypochlorite (NaOCl) irrigation on bacterial reduction and regrowth.
  • To assess the long-term disinfection potential of different instrumentation systems.

Main Methods:

  • Seventy-six human mandibular premolars were infected with E. faecalis.
Keywords:
Enterococcus faecalisMtwoTwisted FileWaveOnebiofilmsregrowth

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  • Root canals were instrumented using Mtwo, Twisted File, or WaveOne systems.
  • Microbiological samples were collected pre- and post-instrumentation, with irrigation using distilled water or 5.25% NaOCl.
  • Bacterial regrowth was monitored for 60 days using Kaplan-Meier survival analysis.

    • Main Results:

    • All systems achieved high initial percentage reduction (95.9%–100%) of E. faecalis, with no significant differences between rotary and reciprocating systems when using either irrigant.
    • In the sodium hypochlorite groups, the Mtwo system showed significantly less bacterial regrowth at 60 days compared to Twisted File (P=0.029) and WaveOne (P=0.005).

    Conclusions:

    • Both rotary and reciprocating systems effectively reduce E. faecalis in root canals.
    • The Mtwo system exhibits superior long-term disinfection efficacy, indicated by reduced bacterial regrowth over 60 days.
    • Sodium hypochlorite irrigation enhances disinfection, but system choice influences long-term outcomes.