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Related Experiment Video

Updated: May 8, 2026

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection

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Two- and three-input TALE-based AND logic computation in embryonic stem cells.

Florian Lienert¹, Joseph P Torella, Jan-Hung Chen

¹Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA and Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.

Nucleic Acids Research

|August 29, 2013

Summary

Researchers developed novel biological computing circuits using split Transcriptional activator-like effectors (TALEs) to perform Boolean logic in mammalian cells. These TALE-based circuits enable complex gene regulation for potential medical applications.

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Published on: August 29, 2020

Area of Science:

Synthetic biology
Molecular biology
Biotechnology

Background:

Biological computing circuits offer precise control over cellular functions.
Transcriptional activator-like effectors (TALEs) are versatile DNA-targeting tools for synthetic gene circuits.

Purpose of the Study:

To demonstrate TALE-based Boolean logic computation in mammalian cells.
To engineer AND logic gates using split-intein protein splicing strategies.

Main Methods:

Utilized split-intein protein splicing to reconstitute functional TALEs from inactive parts.
Implemented two-input and three-input AND logic gates in mammalian cells.
Integrated TALE logic circuits into the mouse embryonic stem cell genome via random and targeted insertion.

Main Results:

Successfully performed two-input AND logic computation using a split-TALE system.
Achieved three-input AND computation through a three-piece intein splicing strategy.
Demonstrated functionality of TALE logic circuits within the mouse embryonic stem cell genome.
Optimized circuit output by modulating TALE-responsive promoter strength.

Conclusions:

Split TALEs are effective tools for constructing logic computation in mammalian cells.
TALE-based logic circuits can control gene expression in response to cellular signals.
This technology holds potential for applications in tissue engineering and medical treatments.