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Surface functionalized thiol-ene waveguides for fluorescence biosensing in microfluidic devices.

Nikolaj A Feidenhans'l1, Josiane P Lafleur, Thomas G Jensen

  • 1Department of Micro- and Nanotechnology, Technical University of Denmark, Kongens Lyngby, Denmark.

Electrophoresis
|August 29, 2013
PubMed
Summary

Thiol-ene polymers enable the simultaneous fabrication of microfluidic channels and optical waveguides. This allows for rapid, site-specific functionalization of waveguides for biosensing applications.

Keywords:
BiosensingFluorescenceMicrofluidicsSurface functionalizationThiol-ene

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Area of Science:

  • Materials Science
  • Optoelectronics
  • Biotechnology

Background:

  • Thiol-ene polymers offer unique physical, optical, and chemical properties suitable for optofluidic device fabrication.
  • Existing methods for creating integrated microfluidic and optical components can be complex and time-consuming.

Purpose of the Study:

  • To develop a streamlined method for fabricating optofluidic devices using thiol-ene polymers.
  • To demonstrate site-specific functionalization of integrated microfluidic channels and optical waveguides for biological applications.

Main Methods:

  • Simultaneous molding of microfluidic channels and optical waveguides using thiol-ene polymers.
  • Photografting of biotin alkyne onto specific waveguide locations using a photomask.
  • Conjugation of fluorescently labeled streptavidin to the photografted biotin alkyne.

Main Results:

  • Thiol-ene polymers were successfully used to create integrated microfluidic channels and optical waveguides in a single molding step.
  • The optical properties of thiol-ene polymers remained consistent across various stoichiometric compositions (refractive index of 1.566 ± 0.008).
  • Site-specific functionalization and subsequent streptavidin conjugation resulted in bright fluorescent patterns at the channel/waveguide interface.

Conclusions:

  • Thiol-ene polymers provide a versatile and efficient platform for fabricating optofluidic devices.
  • The presented method allows for rapid, site-specific biological functionalization of optical waveguides within microfluidic chips.
  • This approach holds promise for developing advanced biosensing and diagnostic tools.