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Updated: May 8, 2026

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
Published on: May 13, 2019
Comparative study of genome divergence in salmonids with various rates of genetic isolation
Elena A Shubina1, Mikhail A Nikitin, Ekaterina V Ponomareva
1Belozersky Institute for Physico-Chemical Biology of Lomonosov Moscow State University, Leninskie gory, 1, Moscow 119991, Russia ; Biological Department of Lomonosov Moscow State University, Leninskie Gory 1, Moscow 119991, Russia.
Abstract:
The aim of the study is a comparative investigation of changes that certain genome parts undergo during speciation. The research was focused on divergence of coding and noncoding sequences in different groups of salmonid fishes of the Salmonidae (Salmo, Parasalmo, Oncorhynchus, and Salvelinus genera) and the Coregonidae families under different levels of reproductive isolation. Two basic approaches were used: (1) PCR-RAPD with a 20-22 nt primer design with subsequent cloning and sequencing of the products and (2) a modified endonuclease restriction analysis. The restriction fragments were shown with sequencing to represent satellite DNA. Effects of speciation are found in repetitive sequences. The revelation of expressed sequences in the majority of the employed anonymous loci allows for assuming the adaptive selection during allopatric speciation in isolated char forms.
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