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Solid-phase microextraction in targeted and nontargeted analysis: displacement and desorption effects.

Sanja Risticevic1, Janusz Pawliszyn

  • 1Department of Chemistry, University of Waterloo , 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1.

Analytical Chemistry
|August 30, 2013
PubMed
Summary

Headspace solid-phase microextraction effectively quantifies complex mixtures using divinylbenzene/Carboxen/poly(dimethylsiloxane) fibers. This method shows optimal extraction for diverse analytes, with minimal displacement issues in food and environmental samples.

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Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Food Chemistry

Background:

  • Headspace solid-phase microextraction (HS-SPME) is crucial for analyzing complex mixtures.
  • Evaluating fiber coatings is essential for accurate quantification in diverse samples.
  • Understanding analyte interactions, like displacement, is key to method reliability.

Purpose of the Study:

  • To define the capabilities and limitations of HS-SPME for multicomponent complex samples.
  • To evaluate commercially available fiber coatings for extraction efficiency and desorption carryover.
  • To investigate coating saturation and interanalyte displacement effects on quantification.

Main Methods:

  • Utilized a complex aqueous mixture with a wide range of volatilities and polarities (log Kow 1.26-8.72).
  • Employed comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC×GC/TOF-MS) to differentiate coatings.
  • Assessed linear dynamic range and displacement effects using spiked samples and apple homogenate with divinylbenzene/Carboxen/poly(dimethylsiloxane) fiber.

Main Results:

  • The divinylbenzene/Carboxen/poly(dimethylsiloxane) fiber demonstrated optimal extraction coverage and sensitivity across a broad analyte range.
  • Interanalyte displacements were infrequent in complex natural samples like apple homogenate.
  • Displacements, when occurring for analytes with small distribution constants, were detectable and correctable by adjusting extraction time.

Conclusions:

  • HS-SPME, particularly with the divinylbenzene/Carboxen/poly(dimethylsiloxane) fiber, is a robust technique for quantifying diverse analytes in complex matrices.
  • The method exhibits high efficiency and sensitivity, with manageable limitations regarding analyte displacement.
  • The findings support the application of HS-SPME in food and environmental analysis, offering strategies to mitigate potential quantification issues.