Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heterogeneous Reactivity of Palladium Nanoparticles Revealed by Wavelength-Resolved Interferometric Scattering.

Nano letters·2026
Same author

Peer Review and AI: Your (Human) Opinion Is What Matters.

ACS nano·2026
Same author

Kinetically Controlled Seed-Mediated Synthesis of Colloidal Copper Nanotetrahedra with Intricate Internal Structure.

Journal of the American Chemical Society·2025
Same author

The Next Ten Years of Nanochemistry: Summary of a Community Workshop on Key Challenges and Opportunities.

ACS nano·2025
Same author

Learning from Metal Nanocrystal Heterogeneity: A Need for Information-Rich and High-Throughput Single-Nanocrystal Measurements.

ACS nanoscience Au·2025
Same author

Scattering vs Interference in Interferometric Scattering Spectroscopy of Plasmonic Nanoparticles.

The journal of physical chemistry letters·2025

Related Experiment Video

Updated: May 8, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Superlocalization surface-enhanced Raman scattering microscopy: comparing point spread function models in the

Eric J Titus1, Katherine A Willets

  • 1Department of Chemistry, The University of Texas at Austin , 105 E. 24th Street STOP A5300, Austin, Texas 78712, United States.

ACS Nano
|August 30, 2013
PubMed
Summary
This summary is machine-generated.

A 3-axis dipole point spread function (PSF) model best fits surface-enhanced Raman scattering (SERS) images of nanoparticle dimers. Dipole models accurately determine molecular orientation, even for single molecules, despite some systematic errors.

More Related Videos

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Highly-Multiplexed Tissue Imaging with Raman Dyes
07:18

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Related Experiment Videos

Last Updated: May 8, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Highly-Multiplexed Tissue Imaging with Raman Dyes
07:18

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Area of Science:

  • Nanophotonics and Spectroscopy
  • Plasmonics
  • Single-molecule imaging

Background:

  • Surface-enhanced Raman scattering (SERS) is a powerful technique for molecular detection.
  • Accurate modeling of point spread functions (PSFs) is crucial for analyzing SERS emission images.
  • Understanding plasmonic coupling in nanoparticle dimers is key to enhancing SERS signals.

Purpose of the Study:

  • To compare the effectiveness of various dipole and Gaussian PSF models for fitting SERS images.
  • To evaluate model performance at both high-concentration and single-molecule limits.
  • To assess the accuracy of molecular orientation determination using different PSF models.

Main Methods:

  • Fitting diffraction-limited SERS emission images of rhodamine 6G-labeled nanoparticle dimers.
  • Utilizing various dipole and Gaussian point spread function (PSF) models.
  • Analyzing data from both high-concentration and single-molecule regimes.

Main Results:

  • A 3-axis dipole PSF model provided the best approximation to experimental PSFs.
  • Dipole models accurately determined dimer geometry and molecular orientation in the high-concentration regime.
  • Single-molecule SERS centroids showed model-dependent mobility, but dipole models retained orientation information.

Conclusions:

  • The 3-axis dipole PSF model is superior for analyzing SERS images of nanoparticle dimers.
  • Dipole models offer reliable orientation data, crucial for understanding plasmonic coupling.
  • While systematic errors exist, dipole PSF models are valuable tools for nanoscale SERS investigations.