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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Related Experiment Video

Updated: May 8, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

What is next generation sequencing?

Sam Behjati1, Patrick S Tarpey

  • 1Cancer Genome Project, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, , Hinxton, Cambridgeshire, UK.

Archives of Disease in Childhood. Education and Practice Edition
|August 30, 2013
PubMed
Summary
This summary is machine-generated.

Next generation sequencing (NGS) rapidly sequences genomes, revolutionizing research. This review explores NGS

Keywords:
Basic ScienceTechnology

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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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Last Updated: May 8, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Published on: August 29, 2014

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

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Published on: September 20, 2016

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Area of Science:

  • Genomics
  • Molecular Biology
  • Pediatric Research

Background:

  • Next generation sequencing (NGS) offers rapid, high-throughput DNA sequencing.
  • NGS technology has significantly advanced genomic research capabilities.
  • Conventional Sanger sequencing is time-intensive for whole genome analysis.

Purpose of the Study:

  • To review the potential applications of Next generation sequencing in pediatrics.
  • To highlight the transition of NGS from research to clinical settings.
  • To discuss the impact of NGS on pediatric genomic studies.

Main Methods:

  • Literature review of Next generation sequencing applications.
  • Analysis of NGS capabilities in genomic research.
  • Exploration of NGS implementation in clinical practice.

Main Results:

  • NGS enables whole human genome sequencing in a single day.
  • NGS has largely replaced Sanger sequencing in research.
  • Clinical adoption of NGS in pediatrics is still emerging.

Conclusions:

  • NGS holds significant promise for pediatric genomic research and diagnostics.
  • Further integration of NGS into routine pediatric clinical practice is anticipated.
  • The review provides insights into the evolving role of NGS in child health.