Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scaled Multidimensional Assays of Variant Effect Identify Sequence-Function Relationships in Hypertrophic Cardiomyopathy.

Circulation·2026
Same author

Genotype-Specific Electrophysiological Remodeling in PLN R14del Cardiomyopathy: Implications for Precision Antiarrhythmic Therapy.

Circulation. Arrhythmia and electrophysiology·2026
Same author

Generation of three induced pluripotent stem cell lines from an immune checkpoint inhibitor-induced myocarditis patient and controls.

Stem cell research·2026
Same author

HeartMap charts new territory in cardiac disease biology.

Nature cardiovascular research·2026
Same author

Indoor air quality and its effects on lung function and respiratory symptoms among textile spinning mill workers in India.

Work (Reading, Mass.)·2026
Same author

Brain Organoids, Lessons from Fetal Neocortex Formation, and Rational Design for Quality Control.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 8, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Developing microRNA screening as a functional genomics tool for disease research.

Derek Lemons1, Mano R Maurya, Shankar Subramaniam

  • 1Department of Bioengineering, Jacobs School of Engineering, University of California San Diego, La Jolla, CA, USA ; Muscle Development and Regeneration Program, Sanford-Burnham Medical Research Institute La Jolla, CA, USA.

Frontiers in Physiology
|August 30, 2013
PubMed
Summary

MicroRNAs (miRNAs) regulate cellular processes and disease. Comprehensive screening of the miRNAome, alongside identifying target proteins, offers a powerful new method for understanding complex biological functions.

Keywords:
functional genomicsfunctional screensmicroRNA targetprotein-protein interactionproteomicssystems biology and network biology

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

Related Experiment Videos

Last Updated: May 8, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • MicroRNAs (miRNAs) were initially identified as regulators of developmental timing in *C. elegans*.
  • miRNAs are now recognized as crucial modulators of diverse cellular processes, impacting both normal development and disease pathogenesis.
  • The genome encodes a limited number of miRNAs, each capable of regulating a substantial portion of the proteome.

Purpose of the Study:

  • To explore the potential of comprehensive miRNAome screening for functional genomics.
  • To investigate the utility of high-throughput screening with miRNA mimics for assay development.
  • To establish a novel approach for gaining insights into complex biological processes through miRNAome analysis and target identification.

Main Methods:

  • Utilizing whole genome libraries of miRNA mimics for high-throughput screening in cell-based assays.
  • Performing comprehensive evaluation of individual miRNA functions.
  • Identifying proteins regulated by specific microRNAs.

Main Results:

  • The advent of miRNA mimic libraries enables large-scale functional screening of the entire miRNAome.
  • This approach allows for the systematic assessment of each miRNA's role in cellular functions.
  • Coupling miRNAome screening with target protein identification provides a powerful strategy for biological discovery.

Conclusions:

  • Comprehensive miRNAome screening represents a significant advancement in functional genomics.
  • This methodology offers a robust platform for dissecting complex biological pathways.
  • Integrating miRNA function and target identification can illuminate fundamental biological mechanisms and disease processes.