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Updated: May 8, 2026

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
Published on: July 23, 2010
HPV18 E1^E4 is assembled into aggresome-like compartment and involved in sequestration of viral oncoproteins
Naoko Kajitani1, Ayano Satsuka, Satoshi Yoshida
1Laboratory of Mammalian Molecular Biology, Department of Molecular and Cellular Biology, Graduate School of Biostudies, Kyoto University Kyoto, Japan ; Laboratory of Gene Analysis, Department of Viral Oncology, Institute for Virus Research, Kyoto University Kyoto, Japan.
Abstract:
Papillomavirus is the etiological agent for warts and several squamous carcinomas. Skin cancer induced by cottontail rabbit papillomavirus was the first animal model for virus-induced carcinogenesis. The target organ of the virus infection is stratified epithelium and virus replication is tightly regulated by the differentiation program of the host cell. E1^E4 protein is a viral gene product, and although it is considered to be involved in the control of virus replication, little is known about the biological role. We found that HPV18 E1^E4 was assembled into an aggresome-like compartment and was involved in sequestration of virus oncoproteins, which might contribute to the differentiation-dependent lifecycle of papillomavirus.
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