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Statistical studies on high molecular weight pullulan production in solid state fermentation using jack fruit seed.

K R Sugumaran¹, R V Sindhu, S Sukanya

¹School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamilnadu, India.

Carbohydrate Polymers

|August 31, 2013

Summary

This study optimized pullulan production using jackfruit seed in solid-state fermentation. Key medium components like KH2PO4 and moisture content significantly boosted polysaccharide yield.

Keywords:

Aureobasidium pullulans Box–Behnken design Plackett–Burman design Pullulan

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Area of Science:

Biotechnology
Microbial Fermentation
Polysaccharide Production

Background:

Pullulan is a versatile polysaccharide with numerous industrial applications.
Utilizing low-cost substrates like jackfruit seed can improve the economic viability of pullulan production.
Solid-state fermentation (SSF) offers advantages for microbial cultivation and product synthesis.

Purpose of the Study:

To optimize medium variables for enhanced pullulan production by Aureobasidium pullulans.
To identify key factors influencing pullulan yield in solid-state fermentation using jackfruit seed.
To characterize the produced pullulan and determine its molecular weight.

Main Methods:

Plackett-Burman design was employed to screen significant medium components (K2HPO4, KH2PO4, ZnSO4·5H2O, MgSO4·7H2O, NaCl, (NH4)2SO4·5H2O, yeast extract, moisture content).
Box Behnken experiment design was used to optimize the screened variables.
Fourier-transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance ((1)H NMR), and carbon-13 nuclear magnetic resonance ((13)C NMR) were used for characterization.
Gel permeation chromatography (GPC) determined the molecular weight.

Main Results:

Medium components significantly affecting pullulan production were identified as KH2PO4, ZnSO4·5H2O, NaCl, and moisture content.
Optimization using Box Behnken design led to maximized pullulan yield.
The produced pullulan was confirmed via spectroscopic methods (FTIR, (1)H NMR, (13)C NMR).
The molecular weight of the synthesized pullulan was determined to be 1.733×10(6) g/mol.

Conclusions:

Jackfruit seed is a cost-effective substrate for pullulan production via Aureobasidium pullulans SSF.
Optimization of specific medium components (KH2PO4, ZnSO4·5H2O, NaCl, moisture) is crucial for maximizing yield.
The characterized pullulan with a high molecular weight holds potential for various applications.