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Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

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High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
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Using high pressure processing (HPP) to pretreat sugarcane bagasse.

J F Castañón-Rodríguez1, B Torrestiana-Sánchez, M Montero-Lagunes

  • 1Instituto Tecnológico de Veracruz, Departamento de Ingenieria Bioquimica - Unidad de Investigación y Desarrollo en Alimentos (UNIDA), Veracruz, Mexico.

Carbohydrate Polymers
|August 31, 2013
PubMed
Summary

High pressure processing (HPP) effectively pretreats sugarcane bagasse, altering its structure and improving enzymatic hydrolysis. HPP combined with NaOH significantly enhances glucose yield, suggesting its potential for biomass conversion.

Keywords:
GlucoseHigh pressure processingHydrolysisPretreatmentSugarcane bagasse

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Area of Science:

  • Biomass Pretreatment
  • Biochemical Engineering
  • Sustainable Materials

Background:

  • Sugarcane bagasse is a lignocellulosic biomass with potential for biofuel and biochemical production.
  • Effective pretreatment is crucial for efficient enzymatic hydrolysis and valorization of bagasse.
  • High pressure processing (HPP) offers a novel approach to modify biomass structure.

Purpose of the Study:

  • To investigate the structural modification of sugarcane bagasse using High Pressure Processing (HPP).
  • To evaluate the impact of HPP parameters (pressure, time, temperature) and chemical additives (acids, NaOH) on bagasse composition and structure.
  • To assess the efficiency of HPP-pretreated bagasse in enzymatic hydrolysis for glucose production.

Main Methods:

  • Sugarcane bagasse was treated using HPP at varying pressures (0-250 MPa), times (5-10 min), and temperatures (25-50 °C).
  • HPP treatments included the addition of phosphoric acid, sulfuric acid, and sodium hydroxide (NaOH).
  • Compositional analysis, structural characterization (XRD), and enzymatic hydrolysis assays were performed.

Main Results:

  • HPP with sulfuric and phosphoric acids degraded significant amounts of hemicellulose (up to 68.62%).
  • HPP with NaOH achieved higher lignin removal (54.10%) and led to structural modifications like cracks and surface fragmentation.
  • HPP treatment combined with 2% NaOH resulted in the highest glucose yield (25 g/L) from enzymatic hydrolysis.

Conclusions:

  • HPP is an effective technology for altering the lignocellulosic structure of sugarcane bagasse.
  • HPP combined with chemical agents, particularly NaOH, enhances biomass susceptibility to enzymatic hydrolysis.
  • HPP pretreatment shows promise for optimizing sugarcane bagasse utilization in biorefineries.