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Updated: May 8, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
Published on: July 5, 2016
Femtosecond digital lensless holographic microscopy to image biological samples
Omel Mendoza-Yero1, Alejandro Calabuig, Enrique Tajahuerce
1Institut de Noves Tecnologies de la Imatge (INIT), Universitat Jaume I, 12071 Castelló, Spain.
Abstract:
The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.
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