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Related Experiment Video

Updated: May 8, 2026

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

Pulsed-light imaging for fluorescence guided surgery under normal room lighting.

Kristian Sexton¹, Scott C Davis, David McClatchy

¹Thayer School of Engineering at Dartmouth College, Hanover, New Hampshire 03755, USA. kristian.j.sexton.th@dartmouth.edu

|August 31, 2013

Summary

This study introduces a novel fluorescence-guided surgery (FGS) system that works in normal room light. This innovation improves surgical workflow by eliminating the need for dim lighting, enhancing visualization of fluorescent markers.

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Area of Science:

Medical technology
Surgical imaging
Biophotonics

Background:

Fluorescence-guided surgery (FGS) enhances surgical outcomes but requires dim lighting, disrupting workflow.
Current FGS systems necessitate dark environments, posing practical challenges in operating rooms.

Purpose of the Study:

To develop a novel FGS system capable of imaging fluorescence under normal room lighting conditions.
To overcome the workflow disruptions associated with traditional dim-light FGS systems.

Main Methods:

A new FGS system utilizing pulsed excitation and gated acquisition was designed.
Performance was evaluated using tissue-simulating phantoms and in vivo orthotopic brain tumor models in mice.

Main Results:

The system achieved visual detection of protoporphyrin IX down to 0.25 μM under 125 μW/cm2 ambient light.
Sensitivity was demonstrated to be over an order of magnitude greater than a commercial FGS system (Zeiss Pentero) operating in the dark.
In vivo studies showed superior sensitivity for detecting orthotopic brain tumors.

Conclusions:

The novel pulsed-light FGS system enables fluorescence imaging under normal room light, significantly improving upon existing technologies.
This advancement offers enhanced sensitivity and overcomes workflow limitations, paving the way for broader adoption of FGS.