Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Charting light harvesting in purple bacteria in vivo.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A serious game for assessing upper-limb visuomotor adaptation in children with cerebral palsy during reaching tasks in virtual reality.

Scientific reports·2026
Same author

Six Months of Bikram Yoga: Longitudinal Effects on Body Fat Reduction and Age-Related Responses in Adult Women.

Healthcare (Basel, Switzerland)·2026
Same author

Selective targeting of NRF2-high pancreatic ductal adenocarcinoma with an NQO1-activatable prodrug.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Nous-209 neoantigen vaccine for cancer prevention in Lynch syndrome carriers: a phase 1b/2 trial.

Nature medicine·2026
Same author

Yoga for Neurodegenerative Disorders: Therapeutic Effects, Mechanisms, and Applications in Alzheimer's and Parkinson's Disease.

Sports (Basel, Switzerland)·2025

Related Experiment Video

Updated: May 8, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Arbitrary-detuning asynchronous optical sampling pump-probe spectroscopy of bacterial reaction centers.

Laura Antonucci1, Adeline Bonvalet, Xavier Solinas

  • 1Laboratoire d’Optique et Biosciences, Ecole Polytechnique, Centre National de la Recherche Scientifique, 91128 Palaiseau, France.

Optics Letters
|August 31, 2013
PubMed
Summary

A new asynchronous optical sampling technique enables pump-probe spectroscopy with a 200 ns time window using a chirped pulse oscillator. This method precisely captures charge transfer dynamics in Rhodobacter sphaeroides reaction centers.

More Related Videos

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
07:55

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

Published on: September 22, 2017

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Related Experiment Videos

Last Updated: May 8, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
07:55

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

Published on: September 22, 2017

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Biophysics

Background:

  • Pump-probe spectroscopy is crucial for studying ultrafast dynamics.
  • Traditional methods often require stabilized lasers, limiting experimental flexibility.
  • Extending the time window is essential for observing slower processes.

Purpose of the Study:

  • To adapt asynchronous optical sampling for pump-probe spectroscopy with unstabilized lasers.
  • To achieve a long time window ( 200 ns) with high time resolution ( 320 fs).
  • To demonstrate the method's capability by studying charge transfer in Rhodobacter sphaeroides.

Main Methods:

  • Utilized a variant of asynchronous optical sampling compatible with arbitrary laser repetition rates.
  • Employed a 5.1 MHz chirped pulse oscillator as the pump laser.
  • Performed pump-probe spectroscopy measurements.

Main Results:

  • Successfully extended the accessible time window to nearly 200 ns.
  • Maintained a time resolution of approximately 320 fs.
  • Measured the complete charge transfer dynamics of Rhodobacter sphaeroides reaction centers in a single experiment.

Conclusions:

  • The adapted asynchronous optical sampling technique significantly enhances pump-probe spectroscopy capabilities.
  • This method allows for the study of slower dynamics previously inaccessible.
  • It provides a powerful tool for investigating complex biological processes like charge transfer.