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Transcription initiation complexes and upstream activation with RNA polymerase II lacking the C-terminal domain of
1Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Molecular and Cellular Biology
|October 1, 1990
Abstract:
RNA polymerase II assembles with other factors on the adenovirus type 2 major late promoter to generate pairs of transcription initiation complexes resolvable by nondenaturing gel electrophoresis. The pairing of the complexes is caused by the presence or absence of the C-terminal domain of the largest subunit. This domain is not required for transcription stimulation by the major late transcription factor in vitro.