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Related Experiment Video

Updated: May 8, 2026

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

Structure of zebrafish MO25.

Zhenzhen Zhang1, Yicui Wang, Zhubing Shi

  • 1School of Life Sciences, Anhui University, Hefei, Anhui 230039, People's Republic of China.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
|August 31, 2013
PubMed
Summary

The MO25 protein structure was determined in zebrafish, revealing conserved functions. This structural insight aids further studies into MO25

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • MO25 is a conserved scaffold protein.
  • MO25 activates the tumor suppressor LKB1 with STRAD.
  • MO25 also enhances STE20-family kinases like MST3, MST4, STK25, SPAK, and OSR1.

Purpose of the Study:

  • To determine the crystal structure of zebrafish MO25.
  • To provide a structural basis for understanding MO25's conserved functions.
  • To facilitate further functional and evolutionary studies of MO25.

Main Methods:

  • Zebrafish MO25 purification and crystallization.
  • X-ray diffraction analysis to determine crystal structure.
  • Molecular replacement method for structure determination.
Keywords:
MO25scaffold proteins

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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Published on: July 17, 2014

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Last Updated: May 8, 2026

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
09:33

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Published on: November 18, 2017

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
06:36

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Published on: July 17, 2014

Main Results:

  • Zebrafish MO25 crystal diffracted to 2.9 Å resolution.
  • The crystal belonged to space group P3221.
  • The determined structure revealed MO25 is composed of seven helical repeats.
  • Structural comparison showed high similarity between zebrafish and human MO25.

Conclusions:

  • Zebrafish MO25 shares structural similarity with human MO25, indicating conserved functions.
  • The determined structure provides a foundation for future research on MO25.
  • This study enhances our understanding of MO25's role in cellular signaling pathways.