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Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

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Published on: January 22, 2020

Response to capacitating stimuli indicates extender-related differences in boar sperm function.

S Schmid¹, H Henning, A M Petrunkina

¹Unit for Reproductive Medicine, Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.

Journal of Animal Science

|August 31, 2013

Summary

This summary is machine-generated.

Boar sperm are sensitive to chilling; a new test measuring their response to capacitation stimuli can detect differences in semen extenders. This method helps identify better preservation strategies for boar semen quality.

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Area of Science:

Reproductive Biology
Animal Science
Sperm Preservation

Background:

Porcine spermatozoa are vulnerable to chilling and aging in vitro.
Developing effective semen extenders is crucial for boar sperm preservation.
Sensitive functional assays are needed to evaluate new preservation strategies.

Purpose of the Study:

To assess if the specific response to capacitation stimuli can detect differences in boar sperm preservation by extenders.
To compare the efficacy of Beltsville Thawing Solution (BTS) and Androstar Plus during hypothermic storage.

Main Methods:

Boar semen was stored in BTS and Androstar Plus at various temperatures (5°C, 10°C, 17°C, 22°C).
Sperm function was assessed using flow cytometry to measure intracellular calcium and plasma membrane integrity after incubation in capacitating and control media.
The specific response to capacitation stimuli was determined by analyzing the loss of low-calcium live cells.

Main Results:

Sperm motility and membrane integrity remained high, except in BTS at 5°C.
Semen stored in BTS showed a greater loss of response to capacitation stimuli compared to Androstar Plus.
Phospholipid disorder showed no significant extender-related differences.
Field insemination trials showed no fertility differences for semen stored up to 48h at 10°C in Androstar Plus versus BTS at 17°C.

Conclusions:

Assessing the specific reactivity to capacitation stimuli is a sensitive method for detecting extender-dependent changes in chilled boar sperm functionality.
Androstar Plus may offer better protection for boar spermatozoa during hypothermic storage compared to BTS.
This functional assay can aid in selecting optimal semen extenders for boar fertility preservation.