Amnibacterium soli sp. nov., an actinobacterium isolated from grass soil

Area of Science:

• Microbiology
• Bacteriology
• Taxonomy

Background:

• Actinobacteria are a diverse phylum of Gram-positive bacteria found in various environments.
• The genus Amnibacterium currently comprises a limited number of characterized species.
• Soil ecosystems harbor a vast, largely uncharacterized microbial diversity.

Purpose of the Study:

• To isolate and characterize a novel actinobacterium from soil.
• To determine the taxonomic affiliation of the novel isolate using polyphasic characterization.
• To propose a new species within the genus Amnibacterium.

Main Methods:

• Isolation and cultivation of bacteria from grass soil.
• 16S rRNA gene sequencing for phylogenetic analysis.
• Phenotypic characterization including cell wall composition, menaquinone analysis, and fatty acid profiling.
• Genomic DNA G+C content determination.

Main Results:

• A Gram-positive, aerobic, non-motile, short rod-shaped actinobacterium, strain PB243(T), was isolated.
• Phylogenetic analysis based on 16S rRNA gene sequences showed highest similarity (98.8%) to Amnibacterium kyonggiense.
• Strain PB243(T) exhibited distinct chemotaxonomic features, including specific menaquinones, diamino acid, and fatty acid profiles.
• The G+C content of the genomic DNA was 71.5 mol%.

Conclusions:

• Strain PB243(T) represents a novel species of the genus Amnibacterium.
• The proposed name for the novel species is Amnibacterium soli sp. nov.
• The type strain is PB243(T) (=KCTC 33147(T) =JCM 19015(T)).