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Updated: May 8, 2026
Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
Published on: May 19, 2019
[Expression of carbonic anhydrase II in cultured rat osteoclasts after stress stimulation]
Qiang Dong1, Qian Xia, Jianjiang Zhou
1Dept. of Stomatology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China.
Objective:
To test expression of carbonic anhydrase II (Ca II) mRNA in osteoclasts which were applied with fluid shear stress.
Methods:
The bone marrow cells of Sprague-Dawley rats were cultured with the presence of 1,25-(OH)2D3 and dexamethasone. The osteoclast-like cells were identified by tartrate-resistant acid phosphatase(TRAP) staining and scanning electron microscope (SEM) observation, then purified with trypsin/ethylenediamine tetraacetic acid (EDTA). Different values and lasting time of steady fluid shear stress were exerted on the osteoclasts with parallel plate flow system. The Ca II expression of osteoclasts were detected by real time reverse transcription-polymerase chain reaction(RT-PCR) and nested polymerase chain reaction(PCR).
Results:
The levels of Ca II mRNA were down-regulated correspondingly with the increase of stress and time (P < 0.05).
Conclusion:
It's indicated that steady fluid shear stress within a certain range may down-regulate the expression of Ca II in osteoclasts.
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