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Mass Analyzers: Common Types

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Related Experiment Video

Updated: May 8, 2026

Experimental Methods for Trapping Ions Using Microfabricated Surface Ion Traps

Experimental Methods for Trapping Ions Using Microfabricated Surface Ion Traps

Published on: August 17, 2017

iTRAQ analysis with Paul ion trap-obstacle solved.

Anna Drabik¹, Anna Bodzoń-Kułakowska, Piotr Suder

¹Department of Biochemistry and Neurobiology, AGH University of Science and Technology , Mickiewicza 30 Ave, 30-059 Kraków, Poland.

Journal of Proteome Research

|September 3, 2013

Summary

Ion trap mass spectrometers can now quantify peptides using isobaric tags (iTRAQ) despite low mass cutoff limitations. This approach enhances proteomic analysis on widely available instruments.

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Experimental Methods for Trapping Ions Using Microfabricated Surface Ion Traps

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Published on: October 25, 2019

Area of Science:

Analytical Chemistry
Biochemistry
Proteomics

Background:

Ion trap mass spectrometers offer high performance and affordability.
A key limitation of ion traps is their low mass cutoff in MS(n) analysis.
This limitation hinders the identification of small molecules and quantification using isobaric tags.

Purpose of the Study:

To present a method for quantitative proteomic analysis using isobaric tags on ion trap mass spectrometers.
To overcome the low mass cutoff limitation of ion traps for specific applications.
To demonstrate the utility of isobaric tags for relative and absolute quantification.

Main Methods:

Utilized isobaric tags for relative and absolute quantification labeling (iTRAQ).
Employed typical, widely available ion trap mass spectrometry devices.
Used manufacturer's software for data analysis.
Performed analyses of standard proteins labeled with isobaric tags at various concentration ratios.

Main Results:

The presented approach enables quantitative proteomic analysis using ion trap devices.
Demonstrated the feasibility of using isobaric tags (iTRAQ) for quantification despite the low mass cutoff.
Series of analyses confirmed the quantitative capabilities of the method.

Conclusions:

The developed approach expands the application of ion trap mass spectrometers in quantitative proteomics.
Isobaric tag labeling (iTRAQ) can be effectively used with ion traps for small molecule and peptide quantification.
This method provides a cost-effective solution for quantitative proteomic studies using common instrumentation.